Article
AU-rich sequences within 5' untranslated leaders enhance translation and stabilize mRNA in Escherichia coli.
Journal of bacteriology - 1 Feb 2005
Komarova Anastassia V, Tchufistova Ludmila S, Dreyfus Marc, Boni Irina V
Abstract excerpt
We have shown previously that when the Escherichia coli chromosomal lacZ gene is put under the control of an extended Shine-Dalgarno (SD) sequence (10 or 6 nucleotides in length), the translation efficiency can be highly variable, depending on the presence of AU-rich targets for ribosomal protein S1 in the mRNA leader. Here, the same strains have been used to examine the question of how strong ribosome binding to...
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