Article
Comparison of selective polymerase chain reaction primers and differential probe hybridization of polymerase chain reaction products for determination of relative amounts of codon 215 mutant and wild-type HIV-1 populations.
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association - 1 Jul 1995
Eastman P S, Urdea M, Besemer D, Stempien M, Kolberg J
Abstract excerpt
A mutation at codon 215 of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) gene results in decreased sensitivity to zidovudine (ZDV). In order to follow changes in codon 215 mutant (MUT) and wild-type (WT) populations in the plasma of patients during therapy, two polymerase chain reaction (PCR) procedures were investigated. The first was a nested, selective PCR, wherein a first round...
Topics
- Base Sequence
- Codon
- DNA Primers
- DNA Probes
- DNA, Single-Stranded
- Didanosine
- Drug Resistance, Microbial
- Drug Therapy, Combination
- Genotype