Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group.

doi:10.1128/JCM.34.7.1849-1853.1996

Article

Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group.

Journal of clinical microbiology - 1 Jul 1996

Shafer R W, Winters M A, Mayers D L, Japour A J, Kuritzkes D R, Weislow O S, White F, Erice A, Sannerud K J, Iversen A, Pena F, Dimitrov D, Frenkel L M, Reichelderfer P S

Abstract excerpt

Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, includi...

Topics

Antiviral Agents
Base Sequence
Codon
DNA Ligases
DNA Primers
DNA, Viral
Drug Resistance, Microbial
Evaluation Studies as Topic
Genotype
HIV Infections

HIV-1

Humans

Laboratories

Leukocytes, Mononuclear

Mutation

Plasma

Polymerase Chain Reaction

Sensitivity and Specificity

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