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Article

Dissecting the energetics of an antibody‐antigen interface by alanine shaving and molecular grafting

1 Dec 1994

Abstract excerpt

Alanine-scanning mutagenesis on human growth hormone (hGH) identified 5 primary determinants (Arg 8, Asn 12, Arg 16, Asp 112, and Asp 116) for binding to a monoclonal antibody (MAb 3) (Jin L, Fendly BM, Wells JA, 1992, J Mol Biol 226:851-865). To further analyze the energetic importance of residues surrounding these five, we mutated all neighboring residues to alanine in groups of 7-16 (a procedure we call...

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PMID: 7538848
DOI: 10.1002/pro.5560031219

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