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Article

Enhancement of Protein-Protein Interactions by Destabilizing Mutations Revealed by HDX-MS.

Biomolecules - 20 Aug 2025

Hamuro Yoshitomo, Armstrong Anthony, Branson Jeffrey, Wu Sheng-Jiun, Huang Richard Y-C, Jacobs Steven

Abstract excerpt

Enhancing protein-protein interactions is a key therapeutic strategy to ensure effective protein function in terms of pharmacokinetics and pharmacodynamics and can be accomplished with methods like directed evolution or rationale design. Previously, two papers suggested the possible enhancement of protein-protein binding affinity via destabilizing mutations. This paper reviews the results of the previous...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 40867645
DOI: 10.3390/biom15081201
PMCID: PMC12384571

Genes

HDX

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

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