Article
Binding of the Grb2 SH2 domain to phosphotyrosine motifs does not change the affinity of its SH3 domains for Sos proline-rich motifs.
The EMBO journal - 1 Sept 1994
Cussac D, Frech M, Chardin P
Abstract excerpt
Phosphotyrosine peptide binding to Grb2 induces tryptophan fluorescence changes in the Src homology 2 (SH2) domain. Affinities are in the nanomolar range, the Shc peptide having the highest affinity, followed by peptides mimicking Grb2 binding sites on EGF and HGF receptors, the putative sites on insulin and IGF-1 receptors having much lower affinities. Proline-rich peptide binding to the SH3 domains induces...
Topics
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Base Sequence
- ErbB Receptors
- GRB2 Adaptor Protein
- Humans
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Peptides
- Phosphotyrosine