Article
Thermodynamics of folding a pseudoknotted mRNA fragment.
Journal of molecular biology - 12 Aug 1994
Gluick T C, Draper D E
Abstract excerpt
A sequence in the leader and first gene of the Escherichia coli alpha mRNA folds into a complex pseudoknot structure that is required for binding of a translational repressor. The thermal denaturation of a 112 nt RNA containing this structure has been followed by calorimetry and UV hyperchromicity. To determine the partially folded intermediates in unfolding, the denaturation of 13 mutants and of several...
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