Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A short 5' splice site RNA oligo can participate in both steps of splicing in mammalian extracts.

RNA (New York, N.Y.) - 1 Oct 1995

Konforti B B, Konarska M M

Abstract excerpt

A short 5' splice site RNA oligonucleotide (5'SS RNA oligo) undergoes both steps of splicing when a second RNA containing the 3' splice site region (3'SS RNA) is added in trans. This trans-splicing reaction displays the same 5' and 3' splice site sequence requirements as cis-splicing of full-length pre-mRNA. The analysis of RNA-snRNP complexes formed on each of the two splice site RNAs is consistent with the...

Topics

Animals
Base Sequence
Binding Sites
Conserved Sequence
HeLa Cells
Humans
Molecular Sequence Data
Mutation
Oligoribonucleotides
RNA Precursors
RNA Splicing
Ribonucleoprotein, U1 Small Nuclear

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Ribonucleoprotein, U2 Small Nuclear

Ribonucleoprotein, U4-U6 Small Nuclear

Source

Open in PubMed Open full text

Identifiers

PMID: 7493327
PMCID: PMC1369322

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7493327-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A short 5' splice site RNA oligo can participate in both steps of splicing in mammalian extracts.PMID 7493327Read ↗

Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.Semantic proximity Read ↗

Mammalian U6 small nuclear RNA undergoes 3' end modifications within the spliceosome.Semantic proximity Read ↗

Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Semantic proximity Read ↗

Mechanism for cryptic splice site activation during pre-mRNA splicing.Semantic proximity Read ↗

The Pre-mRNA Splicing Machinery of Trypanosomes: Complex or Simplified?Semantic proximity Read ↗

Direct interactions between subunits of CPSF and the U2 snRNP contribute to the coupling of pre-mRNA 3' end processing and splicing.Semantic proximity Read ↗

Involvement of U6 snRNA in 5' splice site selection.Semantic proximity Read ↗

Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA.Semantic proximity Read ↗

Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.Semantic proximity Read ↗

U4 small nuclear RNA can function in both the major and minor spliceosomes.Semantic proximity Read ↗

Cotranscriptional spliceosome assembly dynamics and the role of U1 snRNA:5'ss base pairing in yeast.Semantic proximity Read ↗

Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.