Article
Direct interactions between subunits of CPSF and the U2 snRNP contribute to the coupling of pre-mRNA 3' end processing and splicing.
Molecular cell - 21 Jul 2006
Kyburz Andrea, Friedlein Arno, Langen Hanno, Keller Walter
Abstract excerpt
Eukaryotic pre-mRNAs are capped at their 5' ends, polyadenylated at their 3' ends, and spliced before being exported from the nucleus to the cytoplasm. Although the three processing reactions can be studied separately in vitro, they are coupled in vivo. We identified subunits of the U2 snRNP in highly purified CPSF and showed that the two complexes physically interact. We therefore tested whether this interaction...
Topics
- Cleavage And Polyadenylation Specificity Factor
- Escherichia coli
- HeLa Cells
- Humans
- Models, Biological
- Mutation
- Protein Binding
- Protein Subunits
- RNA 3' End Processing
- RNA Precursors
- RNA Splicing
- RNA, Messenger
- Ribonucleoprotein, U2 Small Nuclear
- Substrate Specificity