Article
A covalently trapped folding intermediate of subtilisin E: spontaneous dimerization of a prosubtilisin E Ser49Cys mutant in vivo and its autoprocessing in vitro.
Biochemistry - 18 Jan 1994
Hu Z, Zhu X, Jordan F, Inouye M
Abstract excerpt
The propeptide of subtilisin E (the N-terminal 77 amino acid extension) is required for the proper folding of the nascent mature protein and is also a potent and specific inhibitor of the active enzyme. Previous studies have demonstrated that the propeptide can renature denatured mature sequence...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Circular Dichroism
- Cloning, Molecular
- Enzyme Stability
- Escherichia coli
- Hot Temperature
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed