Article
Mutational analysis of DNase I-DNA interactions: design, expression and characterization of a DNase I loop insertion mutant with altered sequence selectivity.
Protein engineering - 1 Mar 1995
Wolf E, Brukner I, Suck D
Abstract excerpt
A mutant of bovine pancreatic DNase I containing two additional residues in a loop next to C173 has been expressed in Escherichia coli, purified and characterized biochemically. Modelling studies suggest that the inserted arginine and glutamate side chains of the modified loop sequence C173-R-E-G...
Topics
- Animals
- Base Sequence
- Cattle
- DNA
- DNA Mutational Analysis
- DNA-Binding Proteins
- Deoxyribonuclease I
- Escherichia coli
- Genetic Vectors
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Oligodeoxyribonucleotides
- Pancreas
- Protein Binding
- Protein Conformation
- Recombinant Proteins