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Article

Importance of a stable topoisomerase IB clamping for an efficient DNA processing: Effect of the Lys(369)Glu mutation.

International journal of biological macromolecules - 1 Nov 2015

Vieira Sara, Castelli Silvia, Desideri Alessandro

Abstract excerpt

The role of lysine 369 of human topoisomerase IB in recognizing, clamping and processing its DNA substrate was experimentally investigated. Lys(369) is located in one of the two lips that interact to each other allowing the protein to embrace and firmly bind the DNA substrate. The lysine was mutated to a glutamate residue and the catalytic activity of the mutant enzyme was assayed. The mutant shows a distributive...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26231327
DOI: 10.1016/j.ijbiomac.2015.07.044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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