Article
The role of DNA polymerase in base substitution mutagenesis on non-instructional templates.
Biochimie - 1 Jan 2000
Strauss B, Rabkin S, Sagher D, Moore P
Abstract excerpt
In vitro DNA synthesis on phi X174 or M13 templates with non-instructional lesions such as UV dimers or AP (apurinic/apyrimidinic) sites terminates one base before the site of the lesion when synthesis is catalyzed by T4 DNA polymerase or E. coli polymerase I. E. Coli polymerase I also produces termination bands at the site of AP lesions. Substitution of Mn2+ for Mg2+ and increasing the concentration of dNTP's...
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