Article
The role of DNA polymerase in base substitution mutagenesis on non-instructional templates.
Biochimie - 1 Jan 2000
Strauss B, Rabkin S, Sagher D, Moore P
Abstract excerpt
In vitro DNA synthesis on phi X174 or M13 templates with non-instructional lesions such as UV dimers or AP (apurinic/apyrimidinic) sites terminates one base before the site of the lesion when synthesis is catalyzed by T4 DNA polymerase or E. coli polymerase I. E. Coli polymerase I also produces t...
Topics
- Bacteriophage phi X 174
- Base Sequence
- Coliphages
- DNA Polymerase I
- DNA Replication
- DNA-Directed DNA Polymerase
- Escherichia coli
- Kinetics
- Magnesium
- Manganese
- Mutation
- T-Phages
- Templates, Genetic