Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Murine DNA polymerase.alpha-primase initiates RNA-primed DNA synthesis preferentially upstream of a 3'-CC(C/A)-5' motif.

The Journal of biological chemistry - 5 Mar 1990

Davey S K, Faust E A

Abstract excerpt

We find that the purified murine DNA polymerase.alpha-primase complex exhibits the greatest affinity for DNA templates in which CCC occurs 10 nucleotides downstream of a DNA primase initiation site (Km = 6.6 +/- 0.3 pM). Templates with 3'-CCA-5' at this position are less efficiently utilized (Km = 16 +/- 4 pM). Point mutations that disrupt the 3'-CC(C/A)-5' motif further decrease the affinity for DNA...

Topics

Base Sequence
Cloning, Molecular
DNA Primase
DNA Replication
DNA, Viral
Genes, Viral
Minute Virus of Mice
Molecular Sequence Data
Mutation
Nucleotide Mapping
Oligonucleotide Probes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

RNA Nucleotidyltransferases

Restriction Mapping

Templates, Genetic

Source

Identifiers

PMID: 2303465

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2303465-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Murine DNA polymerase.alpha-primase initiates RNA-primed DNA synthesis preferentially upstream of a 3'-CC(C/A)-5' motif.PMID 2303465Read ↗

Arg304 of Human DNA Primase Is a Key Contributor to Catalysis and NTP Binding: Primase and the Family X Polymerases Share Significant Sequence HomologySemantic proximity Read ↗

Flexibility and distributive synthesis regulate RNA priming and handoff in human DNA polymerase α-primaseSemantic proximity Read ↗

Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase.Semantic proximity Read ↗

Crystal structure of the human primase.Semantic proximity Read ↗

Insights into eukaryotic DNA priming from the structure and functional interactions of the 4Fe-4S cluster domain of human DNA primase.Semantic proximity Read ↗

Effects of 3' untranslated region mutations on plus-strand priming during moloney murine leukemia virus replication.Semantic proximity Read ↗

The sequence features important for plus strand priming by human immunodeficiency virus type 1 reverse transcriptase.Semantic proximity Read ↗

Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis.Semantic proximity Read ↗

DNA polymerase fidelity: comparing direct competition of right and wrong dNTP substrates with steady state and pre-steady state kinetics.Semantic proximity Read ↗

The mechanistic architecture of thermostable Pyrococcus furiosus family B DNA polymerase motif A and its interaction with the dNTP substrate.Semantic proximity Read ↗

Characterization of a functional DnaG-type primase in archaea: implications for a dual-primase system.Semantic proximity Read ↗

Functional dissection of a mouse ribosomal protein promoter: significance of the polypyrimidine initiator and an element in the TATA-box region.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.