Article
Mutagenesis by alkylating agents: coding properties for DNA polymerase of poly (dC) template containing 3-methylcytosine.
Biochimie - 1 Jan 2000
Boiteux S, Laval J
Abstract excerpt
After treatment of poly(dC) by the simple alkylating agent (3H)dimethylsulfate, 90 per cent of the radioactivity cochromatographied with 3-methylcytosine and 10 per cent with 5-methylcytosine which is the normally occurring methylated base. In order to study the influence of 3-methylcytosine on D...
Topics
- Alkylating Agents
- Cytosine
- DNA Polymerase I
- DNA Replication
- DNA-Directed DNA Polymerase
- Escherichia coli
- Kinetics
- Magnesium
- Manganese
- Mutation
- Nucleic Acid Hybridization
- Poly C
- Polyribonucleotides
- Templates, Genetic