Article
Mechanism of DNA replication fidelity for three mutants of DNA polymerase I: Klenow fragment KF(exo+), KF(polA5), and KF(exo-).
Biochemistry - 5 Feb 1991
Eger B T, Kuchta R D, Carroll S S, Benkovic P A, Dahlberg M E, Joyce C M, Benkovic S J
Abstract excerpt
Inhibition of the pre-steady-state burst of nucleotide incorporation by a single incorrect nucleotide (nucleotide discrimination) was measured with the Klenow fragment of DNA polymerase I [KF(exo+)]. For the eight mispairs studied on three DNA sequences, only low levels of discrimination ranging from none to 23-fold were found. The kinetics of dNTP incorporation into the 9/20-mer at low nucleotide concentrations...
Topics
- Base Sequence
- DNA Polymerase I
- DNA Replication
- Deoxyribonucleotides
- In Vitro Techniques
- Kinetics
- Manganese
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Oligodeoxyribonucleotides