Article
Template properties of ultraviolet-irradiated poly(dC) replicated by E. coli DNA polymerase I: indication for a role of apyrimidinic-sites in UV-induced mutagenesis.
Biochimie - 1 Jan 2000
Boiteux S, Pierre J, Laval J
Abstract excerpt
Ultraviolet irradiation alters the template properties of poly(dC) when replicated by Escherichia coli DNA polymerase I. These effects are due to base modifications. Some of them are identified as apurinic/apyrimidinic sites (AP-sites) by their sensitivity to AP-endonuclease B purified from Micro...
Topics
- Cytosine
- DNA Polymerase I
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Deoxyadenine Nucleotides
- Deoxyguanine Nucleotides
- Deoxyribonuclease IV (Phage T4-Induced)
- Endodeoxyribonucleases
- Enzyme Activation
- Escherichia coli
- Escherichia coli Proteins
- Macromolecular Substances
- Magnesium
- Manganese
- Mutation
- Poly C
- Polyribonucleotides
- Templates, Genetic
- Ultraviolet Rays