EnzyDiff: Sequence-based classification of mutation-induced enzyme activity direction using latent diffusion denoising.

Abstract excerpt

ObjectivePrediction of mutation-induced enzyme activity changes is important for protein engineering, drug response analysis, and precision medicine. Existing methods often depend on structural information or complex external pipelines, limiting reproducibility and practical use.MethodsWe developed EnzyDiff, a lightweight diffusion-based framework that encodes mutation-induced activity perturbations from enzyme...

Topics

• Enzymes
• Mutation
• Software
• Mutagenesis
• Protein Engineering
• Humans
• Glucosylceramidase