Article
Machine Learning Enables Selection of Epistatic Enzyme Mutants for Stability Against Unfolding and Detrimental Aggregation.
Chembiochem : a European journal of chemical biology - 2 Mar 2021
Li Guangyue, Qin Youcai, Fontaine Nicolas T, Ng Fuk Chong Matthieu, Maria-Solano Miguel A, Feixas Ferran, Cadet Xavier F, Pandjaitan Rudy, Garcia-Borràs Marc, Cadet Frederic, Reetz Manfred T
Abstract excerpt
Machine learning (ML) has pervaded most areas of protein engineering, including stability and stereoselectivity. Using limonene epoxide hydrolase as the model enzyme and innov'SAR as the ML platform, comprising a digital signal process, we achieved high protein robustness that can resist unfolding with concomitant detrimental aggregation. Fourier transform (FT) allows us to take into account the order of the...
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