IMPACT OF FLUORESCENT DYES ON MUTATIONS IN NEXT GENERATION SEQUENCING LIBRARY GENERATION.

Abstract excerpt

DNA labelling fluorescent dyes such as ethidium bromide have long been considered to be highly mutagenic during DNA replication. While recent studies have pushed back on this narrative, the intercalative nature of these dyes continues to raise the possibility that these dyes can induce mutations. The iconPCR instrument by n6tec uses fluorescent dyes to measure amplification in real time and to adjust cycling...

Topics

• Fluorescent Dyes
• High-Throughput Nucleotide Sequencing
• Mutation
• Gene Library
• DNA Mutational Analysis
• Polymerase Chain Reaction