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Article

Highly accurate single-color fluorogenic DNA decoding sequencing for mutational genotyping.

Journal of pharmaceutical and biomedical analysis - 15 Oct 2024

Cheng Chu, Cheng Qingzhou, Zhou Wei, Chen Yulong, Xiao Pengfeng

Abstract excerpt

We proposed a single-color fluorogenic DNA decoding sequencing method designed to improve sequencing accuracy, increase read length and throughput, as well as decrease scanning time. This method involves the incorporation of a mixture of four types of 3'-O-modified nucleotide reversible terminators into each reaction. Among them, two nucleotides are labeled with the same fluorophore, while the remaining two are...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 39111245
DOI: 10.1016/j.jpba.2024.116397

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