Article
Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes.
Nature communications - 17 Aug 2021
Genshaft Alex S, Ziegler Carly G K, Tzouanas Constantine N, Mead Benjamin E, Jaeger Alex M, Navia Andrew W, King Ryan P, Mana Miyeko D, Huang Siyi, Mitsialis Vanessa, Snapper Scott B, Yilmaz Ömer H, Jacks Tyler, Van Humbeck Jeffrey F, Shalek Alex K
Abstract excerpt
A cell's phenotype and function are influenced by dynamic interactions with its microenvironment. To examine cellular spatiotemporal activity, we developed SPACECAT-Spatially PhotoActivatable Color Encoded Cell Address Tags-to annotate, track, and isolate cells while preserving viability. In SPACECAT, samples are stained with photocaged fluorescent molecules, and cells are labeled by uncaging those molecules with...
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