Regulation of N-terminal cleavage and splicing in mini-Ter DnaE-3 intein by E23G and R50Q mutations.

Abstract excerpt

BACKGROUND: As a member of the DnaE intein family, Ter DnaE-3 (TE3) is limited by a relatively low splicing rate, significant undesired N-terminal cleavage reactions, and dependence on native exteins. METHODS: Building on eight previously identified mutation sites from directed evolution, we employed site-directed mutagenesis to systematically investigate how these residues regulate wild-type mini-TE3 splicing...

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