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Article

Intein-Promoted Cyclization of Aspartic Acid Flanking the Intein Leads to Atypical N-Terminal Cleavage.

Biochemistry - 28 Feb 2017

Minteer Christopher J, Siegart Nicolle M, Colelli Kathryn M, Liu Xinyue, Linhardt Robert J, Wang Chunyu, Gomez Alvin V, Reitter Julie N, Mills Kenneth V

Abstract excerpt

Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein, which involves the removal of the intein and the ligation of the flanking polypeptides, or exteins. A DNA polymerase II intein from Pyrococcus abyssi (Pab PolII intein) can promote protein splicing in vitro on incubation at high temperature. Mutation of active site residues Cys1, Gln185, and Cys+1 to Ala results in...

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Identifiers

PMID: 28165720
DOI: 10.1021/acs.biochem.6b00894

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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