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Article

The mechanism of protein splicing and its modulation by mutation.

The EMBO journal - 1 Oct 1996

Xu M Q, Perler F B

Abstract excerpt

Protein splicing results in the expression of two mature proteins from a single gene. After synthesis of a precursor protein, an internal segment (the intein) is excised and the external domains are joined together. A self-catalyzed mechanism for this cleavage-ligation reaction is presented, based on mutagenesis data and analysis of splicing intermediates. Mutations were used to block various steps in the protein...

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Identifiers

PMID: 8895558
PMCID: PMC452257

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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The mechanism of protein splicing and its modulation by mutation.PMID 8895558Read ↗

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Traceless protein splicing utilizing evolved split inteins.Semantic proximity Read ↗

Structural and dynamical features of inteins and implications on protein splicing.Semantic proximity Read ↗

An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile.Semantic proximity Read ↗

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Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation.Semantic proximity Read ↗

Intein-Promoted Cyclization of Aspartic Acid Flanking the Intein Leads to Atypical N-Terminal Cleavage.Semantic proximity Read ↗

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