Article
Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site.
Biochemistry - 10 Jan 2012
Schwarzer Dirk, Ludwig Christina, Thiel Ilka V, Mootz Henning D
Abstract excerpt
Inteins are single-turnover catalysts that splice themselves out of a precursor polypeptide chain. For most inteins, the first step of protein splicing is the formation of a thioester through an N-S acyl shift at the upstream splice junction. However, the mechanism by which this reaction is achieved and the impact of mutations in and close to the active site remain unclear on the atomic level. To investigate...
Topics
- Amino Acid Substitution
- Bacterial Proteins
- Catalysis
- Catalytic Domain
- Cysteine
- DnaB Helicases
- Esters
- Genetic Variation
- Glycine
- Homocysteine
- Inteins
- Molecular Sequence Data