Detection of CRISPR-Cas-induced mutations in Daphnia.

Abstract excerpt

CRISPR-Cas9 has established itself as a robust tool for conducting loss-of-function gene research in emerging model species, including the freshwater zooplankton Daphnia. However, sensitive detection of mutations, especially in genetic mosaic and pooled samples, remains a challenge. In this study, we evaluate 2 of the most widely used mutation screening techniques, the T7 Endonuclease I assay and fragment...

Topics

• Animals
• CRISPR-Cas Systems
• Mutation
• Daphnia
• INDEL Mutation
• Daphnia magna
• Alleles
• Reproducibility of Results