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Energetic Determinants of Expanded PAM Recognition in Engineered FnCas9.

Biochemistry - 18 Aug 2026

Kumar Ajit, Kumari Priyanka, Mishra Shalini, Thakur Shubham, Acharya Sundaram, Chakraborty Debojyoti, Maiti Souvik, Jain Niyati

Abstract excerpt

Expanding protospacer-adjacent motif (PAM) compatibility while preserving specificity remains a central challenge in CRISPR-Cas9 engineering. Francisella novicida Cas9 (FnCas9) exhibits high intrinsic specificity but is constrained by stringent PAM requirements. Here, we quantitatively examine the energetic and catalytic consequences of PAM-interacting mutations in three engineered variants, en1 (E1369R), en15...

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Identifiers

PMID: 42610742
DOI: 10.1021/acs.biochem.6c00350

Genes

PAM

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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