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Article

Redesign of energetically frustrated regions rescues function in defective T4 clamp loaders.

Molecular biology and evolution - 1 Sept 2026

Nimkar Siddharth, Nguyen Thu, Karandur Deepti, Subramanian Subu, O'Donnell Michael E, Kuriyan John

Abstract excerpt

DNA polymerase clamp loaders are AAA + ATPases that load sliding clamps on DNA for high-speed replication. Using a platform for high-throughput mutagenesis of replication proteins in T4 bacteriophage, we carried out saturation mutagenesis of the AAA + ATPase module of the T4 clamp loader bearing a mutation, Gln 118→Asn (Q118N), that reduces fitness. We identified residues for which different mutations improve the...

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Topics

Bacteriophage T4
Adenosine Triphosphatases
Viral Proteins
Mutation
Mutagenesis
DNA-Directed DNA Polymerase

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Identifiers

PMID: 42606177
DOI: 10.1093/molbev/msag204
PMCID: PMC13533335

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