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A DNA polymerase beta mutator mutant with reduced nucleotide discrimination and increased protein stability.

Biochemistry - 25 Sept 2001

Shah A M, Conn D A, Li S X, Capaldi A, Jäger J, Sweasy J B

Abstract excerpt

DNA polymerase beta (pol beta) offers a simple system to examine the role of polymerase structure in the fidelity of DNA synthesis. In this study, the M282L variant of pol beta (M282Lbeta) was identified using an in vivo genetic screen. Met282, which does not contact the DNA template or the incoming deoxynucleoside triphosphate (dNTP) substrate, is located on alpha-helix N of pol beta. This mutant enzyme...

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PMID: 11560485
DOI: 10.1021/bi010755y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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