A DNA polymerase beta mutator mutant with reduced nucleotide discrimination and increased protein stability.

doi:10.1021/bi010755y

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Topics Community

Back to search

Article

A DNA polymerase beta mutator mutant with reduced nucleotide discrimination and increased protein stability.

Biochemistry - 25 Sept 2001

Shah A M, Conn D A, Li S X, Capaldi A, Jäger J, Sweasy J B

Abstract excerpt

DNA polymerase beta (pol beta) offers a simple system to examine the role of polymerase structure in the fidelity of DNA synthesis. In this study, the M282L variant of pol beta (M282Lbeta) was identified using an in vivo genetic screen. Met282, which does not contact the DNA template or the incoming deoxynucleoside triphosphate (dNTP) substrate, is located on alpha-helix N of pol beta. This mutant enzyme...

Topics

Amino Acid Substitution
Base Sequence
DNA Mutational Analysis
DNA Polymerase beta
DNA, Bacterial
Deoxyribonucleotides
Enzyme Stability
Escherichia coli
Frameshift Mutation
Gene Library
Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hot Temperature

Kinetics

Models, Chemical

Models, Molecular

Molecular Sequence Data

Mutation

Nucleic Acid Conformation

Source

Open in PubMed

Identifiers

PMID: 11560485
DOI: 10.1021/bi010755y

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A DNA polymerase beta mutator mutant with reduced nucleotide discrimination and increased protein stability.PMID 11560485Read ↗

Y265H mutator mutant of DNA polymerase beta. Proper teometric alignment is critical for fidelity.Semantic proximity Read ↗

Involvement of phenylalanine 272 of DNA polymerase beta in discriminating between correct and incorrect deoxynucleoside triphosphates.Semantic proximity Read ↗

Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.Semantic proximity Read ↗

Hinge residue Ile260 of DNA polymerase beta is important for enzyme activity and fidelity.Semantic proximity Read ↗

A genetic system to identify DNA polymerase beta mutator mutants.Semantic proximity Read ↗

Human DNA polymerase beta mutations allowing efficient abasic site bypass.Semantic proximity Read ↗

DNA Polymerase β in the Context of CancerSemantic proximity Read ↗

Enzymatic properties of rat DNA polymerase beta mutants obtained by randomized mutagenesis.Semantic proximity Read ↗

Defective Nucleotide Release by DNA Polymerase β Mutator Variant E288K Is the Basis of Its Low Fidelity.Semantic proximity Read ↗

Enzyme-DNA Interactions Required for Efficient Nucleotide Incorporation and Discrimination in Human DNA Polymerase βSemantic proximity Read ↗

Threonine 79 is a hinge residue that governs the fidelity of DNA polymerase beta by helping to position the DNA within the active site.Semantic proximity Read ↗

The E288K colon tumor variant of DNA polymerase β is a sequence specific mutator.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.