Article
Intragenic suppressors of Dictyostelium myosin G680 mutants demarcate discrete structural elements. Implications for conformational states of the motor.
Genetics - 1 Aug 1998
Patterson B
Abstract excerpt
We are using intragenic suppression to generate inferences about currently inaccessible conformations of the molecular motor myosin. We identified 19 unique suppressors of Dictyostelium G680A and G680V mutants and find that they are structurally and chemically restricted, suggestive of specific,...
Topics
- Animals
- Base Sequence
- Biomechanical Phenomena
- Cold Temperature
- DNA Primers
- Dictyostelium
- Genes, Fungal
- Genes, Protozoan
- Models, Biological
- Models, Molecular
- Myosins
- Phenotype
- Point Mutation
- Polymerase Chain Reaction
- Protein Conformation
- Suppression, Genetic