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Article

Long-Range Destabilizing Effects of Mutations at the Escherichia coli β Clamp Dimer Interface.

Biochemistry - 15 Jul 2025

Liriano Melissa L, Berger Madison B, Nguyen Kenny, Korzhnev Dmitry M, Cisneros G Andrés, Beuning Penny J

Abstract excerpt

The bacterial processivity factor, the β sliding clamp, endows Pol III DNA polymerase α with efficiency and processivity during DNA replication by tethering α to DNA. The dimeric β clamp is loaded onto primer:template DNA junctions by a five-subunit clamp loader complex. We previously showed that tuning the stability of the β dimer interface affected clamp opening dynamics and function. The L82D clamp mutation...

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Identifiers

PMID: 40574562
DOI: 10.1021/acs.biochem.5c00135
PMCID: PMC12269063

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