PAM‐relaxed and temperature‐tolerant CRISPR‐Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato

Abstract excerpt

Summary Class 2 Type V‐A CRISPR‐Cas (Cas12a) nucleases are powerful genome editing tools, particularly effective in A/T‐rich genomic regions, complementing the widely used CRISPR‐Cas9 in plants. To enhance the utility of Cas12a, we investigate three Cas12a orthologs—Mb3Cas12a, PrCas12a, and HkCas12a—in plants. Protospacer adjacent motif (PAM) requirements, editing efficiencies, and editing profiles are compared...

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