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Article

ATP-Red enables single-cell bioenergetic phenotyping by flow cytometry.

Cell reports methods - 21 Sept 2026

Marin Jose, Singh Bharati, Fuchs Amanda, Tarasenko Tatiana, Warren Emily, Kirby Martha, Anderson Stacie, Wincovitch Stephen M, Gordon-Lipkin Eliza M, Kruk Shannon, West A Phillip, McGuire Peter J

Abstract excerpt

Most established bioenergetic assays lack single-cell resolution and may mask metabolic heterogeneity. The main flow-cytometry-based approach for bioenergetic analysis infers energetic state from protein synthesis, although translation can become uncoupled from ATP availability under physiologic and pathologic conditions. Here, we evaluated ATP-Red as a flow-cytometry-compatible readout of energetic state at...

Read the complete abstract on PubMed

Topics

Flow Cytometry
Energy Metabolism
Adenosine Triphosphate
Single-Cell Analysis
Humans
Phenotype

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Mitochondria

Animals

Glycolysis

Source

Open in PubMed Open full text

Identifiers

PMID: 42532047
DOI: 10.1016/j.crmeth.2026.101537
PMCID: PMC13615503

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 23 Oct 2026.

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