Why a Single Mutation Dims the Light: Local Chromophore Organization Modulates Fluorescence in smURFP.

Abstract excerpt

Predicting mutation-induced photophysical changes in bilin-binding fluorescent proteins remains challenging. Here, we investigate the origin of fluorescence modulation in smURFP and its low-fluorescent-quantum-yield Y56R variant using classical MD simulations and QM/MM calculations. Comparison of multiple chromophore binding states supports a wild-type configuration involving A-ring attachment and chromophore...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Fluorescence
• Share to Light
• Share to Luminescent Proteins