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Article

Conformational Dynamics of mCherry Variants: A Link between Side-Chain Motions and Fluorescence Brightness.

The journal of physical chemistry. B - 12 Jan 2023

Mukherjee Srijit, Manna Premashis, Douglas Nancy, Chapagain Prem P, Jimenez Ralph

Abstract excerpt

The 3-fold higher brightness of the recently developed mCherry-XL red fluorescent protein (FP) compared to its progenitor, mCherry, is due to a significant decrease in the nonradiative decay rate underlying its increased fluorescence quantum yield. To examine the structural and dynamic role of the four mutations that distinguish the two FPs and closely related variants, we employed microsecond time scale,...

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Identifiers

PMID: 36574626
DOI: 10.1021/acs.jpcb.2c05584

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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