Article
Bright blue-shifted fluorescent proteins with Cys in the GAF domain engineered from bacterial phytochromes: fluorescence mechanisms and excited-state dynamics.
Scientific reports - 18 Nov 2016
Hontani Yusaku, Shcherbakova Daria M, Baloban Mikhail, Zhu Jingyi, Verkhusha Vladislav V, Kennis John T M
Abstract excerpt
Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are of great interest for in vivo imaging. They utilize biliverdin (BV) as a chromophore, which is a heme degradation product, and therefore they are straightforward to use in mammalian tissues. Here, we report on fluorescence properties of NIR FPs with key alterations in their BV binding sites. BphP1-FP, iRFP670 and...
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