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Article

Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.

Biomolecules - 8 Dec 2020

Samson Camille, Legrand Pierre, Tekpinar Mustafa, Rozenski Jef, Abramov Mikhail, Holliger Philipp, Pinheiro Vitor B, Herdewijn Piet, Delarue Marc

Abstract excerpt

Archaeal DNA polymerases from the B-family (polB) have found essential applications in biotechnology. In addition, some of their variants can accept a wide range of modified nucleotides or xenobiotic nucleotides, such as 1,5-anhydrohexitol nucleic acid (HNA), which has the unique ability to selectively cross-pair with DNA and RNA. This capacity is essential to allow the transmission of information between...

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Identifiers

PMID: 33302546
DOI: 10.3390/biom10121647
PMCID: PMC7763228

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