Article
U2AF1 mutations rescue deleterious exon skipping induced by KRAS mutations.
Nature genetics - 1 Jul 2026
Walter David M, Cho Katherine, Sivakumar Smruthy, Denney Daniel, Lee Iris T, Dohlman Anders B, Heinz Jakob M, Shurberg Ethan, Jiang Kevin X, Gupta Akansha A, Frampton Garrett M, Meyerson Matthew
Abstract excerpt
The mechanisms by which mutations of splicing factor gene U2AF1 contribute to lung adenocarcinoma pathogenesis are not well understood. Here we used prime editing to modify the endogenous U2AF1 gene in lung adenocarcinoma cells and assessed the impact on alternative splicing. One specific KRAS mutation, G12S, led to skipping of KRAS exon 2 and generation of a nonfunctional KRAS transcript. However, expression of...
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