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Article

A massively parallel pipeline to clone DNA variants and examine molecular phenotypes of human disease mutations.

PLoS genetics - 1 Dec 2014

Wei Xiaomu, Das Jishnu, Fragoza Robert, Liang Jin, Bastos de Oliveira Francisco M, Lee Hao Ran, Wang Xiujuan, Mort Matthew, Stenson Peter D, Cooper David N, Lipkin Steven M, Smolka Marcus B, Yu Haiyuan

Abstract excerpt

Understanding the functional relevance of DNA variants is essential for all exome and genome sequencing projects. However, current mutagenesis cloning protocols require Sanger sequencing, and thus are prohibitively costly and labor-intensive. We describe a massively-parallel site-directed mutagenesis approach, "Clone-seq", leveraging next-generation sequencing to rapidly and cost-effectively generate a large...

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Identifiers

PMID: 25502805
DOI: 10.1371/journal.pgen.1004819
PMCID: PMC4263371

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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