Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.

BMC research notes - 19 Jun 2026

Li Zidan, Al'Abri Ibrahim, Crook Nathan

Abstract excerpt

OBJECTIVE: The purpose of this project was to evaluate the performance and reliability of the P1 phage-based inducible directed evolution (IDE) method for performing global transcriptional machinery engineering (gTME) to improve pH tolerance in Escherichia coli Nissle. Unlike prior gTME approaches that evolved single sigma factors, we simultaneously mutated three sigma factors associated with pH tolerance (rpoD,...

Read the complete abstract on PubMed

Topics

Directed Molecular Evolution
Escherichia coli
Sigma Factor
Bacteriophage P1
Hydrogen-Ion Concentration
Mutation
Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Plasmids

Gene Deletion

Source

Open in PubMed Open full text

Identifiers

PMID: 42321846
DOI: 10.1186/s13104-026-07913-4
PMCID: PMC13528150

Genes

IDE

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 23 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.PMID 42321846Read ↗

Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.Semantic proximity Read ↗

Inducible directed evolution of complex phenotypes in bacteria.Semantic proximity Read ↗

Engineered Hypermutation Adapts Escherichia coli to Octanoic Acid Stress.Semantic proximity Read ↗

Phenotypic consequences of RNA polymerase dysregulation in Escherichia coli.Semantic proximity Read ↗

Host evolution improves genetic circuit function in complex growth environmentsSemantic proximity Read ↗

Engineering 2,4-Diacetylphloroglucinol-Hyper-Tolerant Escherichia coli via Adaptive Evolution for Efficient Biosynthesis and an Organic Solvent-Free Downstream Process.Semantic proximity Read ↗

Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes.Semantic proximity Read ↗

Negative selection and stringency modulation in phage-assisted continuous evolution.Semantic proximity Read ↗

Phage encoded H-NS: a potential achilles heel in the bacterial defence system.Semantic proximity Read ↗

Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds.Semantic proximity Read ↗

Rapid and Continuous Directed Evolution in Vibrio natriegens Utilizing an In Vivo Hypermutation System.Semantic proximity Read ↗

Assessing the potential of mutational strategies to elicit new phenotypes in industrial strains.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.