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Article

Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.

Nature microbiology - 1 Jun 2026

Ong Shujian, Ghode Pramila, Narenderan Ashvinath, Lao Shuxuan, Willenborg Fabian, Eden Tobias V, Marsh Carl O, Yew Wen Shan, Fredens Julius

Abstract excerpt

Directed evolution methods face trade-offs between the control of discrete approaches and the throughput of modern continuous systems. Here, we engineered a method called lytic selection and evolution (LySE) for near-continuous evolution of bacterial gene clusters while maintaining discrete checkpoints. We developed a hypermutagenic T7 DNA polymerase variant fused to a dual adenine-cytosine deaminase to install...

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Identifiers

PMID: 42067623
DOI: 10.1038/s41564-026-02346-y
PMCID: PMC13236587

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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