Negative selection and stringency modulation in phage-assisted continuous evolution.

Abstract excerpt

Phage-assisted continuous evolution (PACE) uses a modified filamentous bacteriophage life cycle to substantially accelerate laboratory evolution experiments. In this work, we expand the scope and capabilities of the PACE method with two key advances that enable the evolution of biomolecules with radically altered or highly specific new activities. First, we implemented small molecule-controlled modulation of...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophages
• Share to Biological Evolution
• Share to DNA-Directed RNA Polymerases