Article
Improving the Activity of Phi29 DNA Polymerase by SaProt Prediction.
Chemistry (Weinheim an der Bergstrasse, Germany) - 19 Sept 2026
Zhu Qian, Liu Mengqi, Peng Xiaohan, Zhou Chunlan, Gong Yeqin, Zhang Aojia, Chen Shiyu, Zhou Jiayi, Zhang Meizhou, Wu Yuzhou, Yang Ni
Abstract excerpt
Phi29 DNA polymerase has broad application value due to its excellent functions such as strand displacement. However, the catalytic efficiency of the natural Phi29 DNA polymerase is low, which greatly limits its application scope. This study attempts to modify Phi29 DNA polymerase using the deep prediction model SaProt to improve its catalytic efficiency. First, the 3D structure of Phi29 DNA polymerase was...
Read the complete abstract on PubMedTopics
- DNA-Directed DNA Polymerase
- Mutagenesis, Site-Directed
- Models, Molecular
- Bacillus Phages
- Mutation