Molecular insights into particular mutations impact on Taq polymerase dynamics and structure: a molecular dynamics simulation study.

Abstract excerpt

Taq DNA polymerase (Taq pol), a useful enzyme in biotechnology, has been the subject of an extensive investigation to create enzymes with altered characteristics, notably the enhancement of Taq pol's thermostability, But the demand for such enzymes remains unmet. In this study, we aimed to introduce several mutations to the Taq pol structure to improve its structural stability. We combined the sequence-based and...

Topics

• Molecular Dynamics Simulation
• Mutation
• Hydrogen Bonding
• Taq Polymerase
• Protein Conformation
• Enzyme Stability
• Protein Structure, Secondary