Article
Customized multiple sequence alignment as an effective strategy to improve performance of Taq DNA polymerase.
Applied microbiology and biotechnology - 1 Nov 2023
Li Xinjia, Chen Binbin, Chen Wanyi, Pu Zhongji, Qi Xin, Yang Lirong, Wu Jianping, Yu Haoran
Abstract excerpt
Engineering Taq DNA polymerase (TaqPol) for improved activity, stability and sensitivity was critical for its wide applications. Multiple sequence alignment (MSA) has been widely used in engineering enzymes for improved properties. Here, we first designed TaqPol mutations based on MSA of 2756 sequences from both thermophilic and non-thermophilic organisms. Two double mutations were generated including a variant...
Topics
- Molecular Dynamics Simulation
- Sequence Alignment
- Taq Polymerase
- Enzyme Stability
- Protein Engineering
- Mutation