Article
Insights into strand displacement and processivity from the crystal structure of the protein-primed DNA polymerase of bacteriophage phi29.
Molecular cell - 19 Nov 2004
Kamtekar Satwik, Berman Andrea J, Wang Jimin, Lázaro José M, de Vega Miguel, Blanco Luis, Salas Margarita, Steitz Thomas A
Abstract excerpt
The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides explanations for its extraordinary processivity and strand displacement activities. Homology modeling...
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