Article
A multiplexed electrochemical quantitative polymerase chain reaction platform for single-base mutation analysis.
Biosensors & bioelectronics - 15 Oct 2022
Wang Yang, Sun Hong, Xu Gaolian, Guan Mengdi, Zhang Qingyang, Wang Zhiying, Dong Zaizai, Chen Wenhui, Yang Xiaoxiao, Qiao Anbang, Fan Yubo, Cai Xinxia, Chen Zhou, Chang Lingqian, Wei Bo
Abstract excerpt
Detection of single-based mutation (SbM), which is of ultra-low abundance against wild-type alleles, are typically constrained by the level of multiplexing, sensitivity for single-base resolution and quantification accuracy. In this work, an electrochemical quantitative polymerase chain reaction (E-PCR) platform was developed for multiplexed and quantitative SbM analysis in limited and precious samples with...
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