Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

SuperSelective PCR primers for determining cis/trans configurations of mutations within the same gene.

PloS one - 1 Jan 2026

Vargas Diana Yaneth, Kramer Fred Russell

Abstract excerpt

In non-small cell lung cancer, the choice of an effective targeted therapy often depends on the presence of a somatic mutation that alters an amino acid in a key protein. However, if an additional somatic mutation occurs in the same mutant gene, causing an additional amino acid substitution to occur in that protein, the targeted therapy can become ineffective. A PCR assay has now been developed that utilizes two...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Carcinoma, Non-Small-Cell Lung
Share to DNA Mutational Analysis
Share to DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to ErbB Receptors

Share to Lung Neoplasms

Share to Mutation

Share to Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 42149869
DOI: 10.1371/journal.pone.0349389
PMCID: PMC13183233

Genes

EGFR

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

SuperSelective PCR primers for determining cis/trans configurations of mutations within the same gene.PMID 42149869Read ↗

Multiplex SuperSelective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations in Liquid Biopsies.Semantic proximity Read ↗

SuperSelective primer pairs for sensitive detection of rare somatic mutations.Semantic proximity Read ↗

Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer.Semantic proximity Read ↗

Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations.Semantic proximity Read ↗

Custom-Designed Probes for the Accurate Determination of Epidermal Growth Factor Receptor Mutations and Their Allelic Configuration.Semantic proximity Read ↗

Most T790M mutations are present on the same EGFR allele as activating mutations in patients with non-small cell lung cancer.Semantic proximity Read ↗

Ultra-sensitive monitoring of leukemia patients using superRCA mutation detection assays.Semantic proximity Read ↗

PCR-based testing for therapy-related EGFR mutations in patients with non-small cell lung cancer.Semantic proximity Read ↗

Detection of basepair substitution mutation at a frequency of 1 x 10(-7) by combining two genotypic selection methods, MutEx enrichment and allele-specific competitive blocker PCR.Semantic proximity Read ↗

Kinetic hairpin oligonucleotide blockers for selective amplification of rare mutations.Semantic proximity Read ↗

Multiplex DNA amplification and solid-phase direct sequencing for mutation analysis at the hprt locus in Chinese hamster cells.Semantic proximity Read ↗

The use of EGFR exon 19 and 21 unlabeled DNA probes to screen for activating mutations in non-small cell lung cancer.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.