Article
Multiplex SuperSelective PCR Assays for the Detection and Quantitation of Rare Somatic Mutations in Liquid Biopsies.
The Journal of molecular diagnostics : JMD - 1 Mar 2022
Vargas Diana Y, Tyagi Sanjay, Marras Salvatore A E, Moerzinger Patricia, Abin-Carriquiry Juan A, Cuello Mauricio, Rodriguez Clara, Martinez Andrés, Makhnin Alex, Farina Andrea, Patel Chintan, Chuang Tuan L, Li Bob T, Kramer Fred R
Abstract excerpt
SuperSelective primers, by virtue of their unique design, enable the simultaneous identification and quantitation of inherited reference genes and rare somatic mutations in routine multiplex PCR assays, while virtually eliminating signals from abundant wild-type sequences closely related to the target mutations. These assays are sensitive, specific, rapid, and low cost, and can be performed in widely available...
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